The Relationship between Blood MDA Levels and Aortic Foam Cells in Menopausal Wistar Rats Treated with Cempaka Flower Extract

Authors

  • Lely firrahmawati Universitas Aisyiyah Surakarta
  • Endang Sri Wahyuni Universitas Aisyiyah Surakarta
  • M. Mulyaningsih Universitas Aisyiyah Surakarta
  • Prihani Sandra Widyanti Universitas Aisyiyah Surakarta

DOI:

https://doi.org/10.30787/gaster.v19i2.721

Keywords:

Cempaka, MDA, Sel Busa, Menopause

Abstract

Menopause is the natural end of the menstrual cycle which results in decreased estrogen (hypoestrogen) levels. The number of free radicals can increase due to decreased estrogen as a natural antioxidant in the body. The effect of increasing free radicals can be reduced by consuming antioxidants. Cempaka flower (Michelia champaca L) contains flavonoids which function as antioxidants. Materials and methods: proved a relationship between blood MDA levels and aortic foam cells in menopausal wistar rats treated with Cempaka flower extract. Research Methods: using experimental design with post test only control group design approach. The sample used 24 female rats (Rattus novergicus strain Wistar) with a menopausal age (450-540 days). Reasult: The normality assumption test with Saphiro Wilk obtained a p-value of 0.537 more than α = 0.05 (p> 0.05). The homogeneity test of variance with the Levene test obtained a p-value of 0.271 more than 0.05 (p> 0.05). Testing the effect of giving Cempaka flower extract on MDA levels using ANOVA obtained a p-value of 0.000 smaller than α = 0.05 (p <0.05). There was a significant difference in MDA levels due to the administration of cempaka flower extract at different doses. Testing the effect of giving Cempaka flower extract on the number of foam cells with the Kruskal-Wallis test obtained a p-value of 0.004, smaller than α = 0.05 (p <0.05). There is a significant difference in the number of foam cells due to the administration of Cempaka Flower extract with different doses. Testing the correlation between MDA levels and the number of foam cells used the Spearman correlation test with a correlation coefficient of 0.380 and a p-value of 0.067. P-value more than 0.05 (p> 0.05). Conclusion: There is a significant effect of giving Cempaka flower extract on blood MDA levels and aortic foam cells of menopausal wistar rats. There was no significant relationship between blood MDA levels and the number of aortic foam cells.

Author Biographies

Lely firrahmawati, Universitas Aisyiyah Surakarta

MIDWIVERY DEPARTEMEN

Endang Sri Wahyuni, Universitas Aisyiyah Surakarta

MIDWIVERY DEPARTEMEN

M. Mulyaningsih, Universitas Aisyiyah Surakarta

MANAGEMEN NURSING

Prihani Sandra Widyanti, Universitas Aisyiyah Surakarta

MIDWIVERY STUDENT

References

Ashok Agarwal (2012) ‘The effects of oxidative stress on female reproduction: a review’, Reproductive biology and endocrinology, 10(1), p. 49. doi: 10.6036/8204.

Chainy, G. B. N. and Sahoo, D. K. (2020) ‘Hormones and oxidative stress: an overview’, Free Radical Research. Taylor & Francis, 54(1), pp. 1–26. doi: 10.1080/10715762.2019.1702656.

Doshi, Sejal B., and A. A. " (2013) ‘The role of oxidative stress in menopause’, Journal of mid-life health, 4(3), p. 140.

Fajria Maulida, L. and Sri Wahyuni, E. (2018) ‘Upaya Menurunkan Radikal Bebas Dengan Ekstrak Bunga Cempaka Pada Tikus Model Menopause’, Gaster, 16(1), p. 6. doi: 10.30787/gaster.v16i1.238.

Fazdria and Ishak, S. (2020) ‘Efek Protektif Michelia Champaca L Pada Vaskular Tikus Usia Menopause’, Journal of Healthcare Technology and Medicine, 6(2), p. 1037. doi: 10.33143/jhtm.v6i2.1090.

Fritz, M. A., and L. S. (2011) ‘Long-acting methods of contraception’, Clinical gynecologic endocrinology and infertility, 8, pp. 1059–93.

Gowda, Amoolya, et al (2014) ‘The Wound Healing Property of Ethanolic Extract of Michelia Champaca Flowers in Diabetic Rats’, Int J Basic Clin Pharmacol, 3(6), pp. 1036–1042.

Jideani, A. I. O. et al. (2021) ‘Antioxidant-rich natural fruit and vegetable products and human health’, International Journal of Food Properties. Taylor & Francis, 24(1), pp. 41–67. doi: 10.1080/10942912.2020.1866597.

Khan, S. A. and Priyamvada, S. (2018) ‘Green Tea Consumption Ameliorates Intestinal and Hepatic-Toxicity induced by Long-Term administration of Cisplatin’, Int J Drug Metab Toxicol, 02(01), pp. 01–09. Available at: https://orcid.org/0000-0002-0870-.

Muchtadi, D. (2013) Antioksidan & Kiat Sehat Di Usia Produktif. Bandung: Alfabeta.

Murray, R. K., Granner, D. K. & Rodwell, V. W. (2012) Biokimia Harper. Jakarta: Penerbit Buku Kedokteran EGC.

Parsanezhad, M. E. et al. (2013) ‘Pregnancy rate after endometrial injury in couples with unexplained infertility: A randomized clinical trial’, International Journal of Reproductive BioMedicine, 11(11), pp. 869–874.

Partiwisari, N. et al (2014) ‘Identifikasi Simplisia Kulit Batang Cempaka Kuning (Michelia Champaca L.) Secara Makroskopis Dan Mikroskopis’, Jurnal Farmasi Udayana, 3(2), p. 279786.

Pusparatri, E. et al (2020) ‘HUBUNGAN ANTARA PENERIMAAN DIRI DENGAN KECEMASAN’, Indonesia Jurnal Perawat, 1(1), pp. 22–30.

Situmorang, N., Utara, U. S. and Utara, S. (2020) ‘Malondialdehyde (mda)’, 2(2).

Sullivan, Shannon D., Philip M. Sarrel, and L. M. N. (2016) ‘Hormone replacement therapy in young women with primary ovarian insufficiency and early menopause’, Fertility and sterility, 106(7), pp. 1588-1599.

Veri, N. et al. (2021) ‘Terhadap Sel Endotel Pembuluh Darah Pada Tikus’, 7(2), pp. 149–154.

Wahyuni, E. S. and Purwaningsih, W. (2016) ‘Kombinasi Vitamin E Dan C Menurunkan Diameter Sel Lemak’, Prosiding Seminar Nasional & Internasional, 1(1).

Wend, K., Wend, P. and Krum, S. A. (2012) ‘Tissue-specific effects of loss of estrogen during menopause and aging’, Frontiers in Endocrinology, 3(FEB), pp. 1–14. doi: 10.3389/fendo.2012.00019.

Yeh, Y. T. et al. (2011) ‘Bioactive constituents from Michelia champaca’, Natural Product Communications, 6(9), pp. 1251–1252. doi: 10.1177/1934578x1100600910.

Published

2021-08-30

How to Cite

firrahmawati, L., Wahyuni, E. S., Mulyaningsih, M., & Widyanti, P. S. (2021). The Relationship between Blood MDA Levels and Aortic Foam Cells in Menopausal Wistar Rats Treated with Cempaka Flower Extract. Gaster, 19(2), 183–194. https://doi.org/10.30787/gaster.v19i2.721

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